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Indian J Exp Biol ; 2000 Mar; 38(3): 293-6
Article in English | IMSEAR | ID: sea-61051

ABSTRACT

Protease was isolated from Sporosarcina RRLJ1 which was collected from acid tea (Camellia sinensis) plantations. It showed potential for production of the enzyme for commercial purposes. The study revealed that optimum pH for growth of the organism was 6.5-7 and supplement of casein (1%) in the medium was required for production of protease. Enzyme production and enzyme activity was maximum in 72 hr old broth culture. Maximum activity of the enzyme was found at pH 6.5.


Subject(s)
Culture Media , Endopeptidases/isolation & purification , Gram-Positive Endospore-Forming Bacteria/enzymology , Hydrogen-Ion Concentration , Tea/microbiology
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